Possible mechanisms of action of an anti-Pasteurella pestis factor.

نویسندگان

  • D M Eisler
  • R J Heckly
چکیده

Anti-Pasteurella pestis factor (APF) inhibited bacterial growth, but there was no evidence that APF from either mouse or guinea pig or selected fatty acids physically disrupted the cell wall. The fatty acids selected were representative of those found in APF. APF inhibited oxidation of beta-d-glucose but not oxidation of glucose-6-phosphate, whereas fatty acids inhibited the oxidation of glucose-6-phosphate but not oxidation of beta-d-glucose. The oxidation of 6-phosphogluconic acid was inhibited by both APF and free fatty acids. Furthermore, APF and potassium laurate inhibited 6-phosphogluconic dehydrogenase in a cell-free extract of P. pestis strain E.V. 76. No evidence of beta-d-glucose or glucose-6-phosphate dehydrogenases was found in the cell-free extract. The results suggested that APF and fatty acids may kill P. pestis by inactivating 6-phosphogluconic acid dehydrogenase. The effects of these agents on other enzyme systems were not excluded.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Demonstration of blood group substance A bound to Pasteurella pestis.

DEMONSTRATION OF BLOOD GROUP SUBSTANCE A BOUND TO PASTEURELLA PESTIS OBJECTIVE To determine the source, occurrence and location of human blood group antigens in P. pestis vaccines, and to quantitate the hemolytic potency of rabbit anti-P. pestis sera for human type A, B and 0 red cells.

متن کامل

The Action of Pasteurella pestis Bacteriophage on Strains of Pasteurella, Salmonella, and Shigella.

Bacteriophages active against Pasteurella pestis have been described by a number of workers, including Flu (1927), Sugino (1932), Advier (1933), and Girard (1942). The subject has been reviewed by Harvey (1933) and Wu et al. (1936). This report describes certain relationships of P. pestis to Pasteurella pseudotuberculosis and to Salmonella and Shigella strains, as shown by bacteriophage action ...

متن کامل

Bacteriophage Common to Pasteurella Pestis and Escherichia Coli.

Hertman, I. (Israel Institute for Biological Research, Ness Ziona, Israel). Bacteriophage common to Pasteurella pestis and Escherichia coli. J. Bacteriol. 88:1002-1005. 1964.-Phage Y of Pasteurella pestis and phage T(3) of Escherichia coli are serologically related and have common bacterial hosts. Anti-Y serum neutralizes the homologous phage and phage T(3) to a similar extent. Anti-Y serum, ab...

متن کامل

Pasteurella pestis: Role of Pesticin I and Iron in Experimental Plague.

Loss of the genetic determinant for pesticin I in Pasteurella pestis results in concomitant loss of the plague coagulase and fibrinolytic factor. The median lethal dose for mice of an isolate lacking only these activities is increased by factors of about 10(1), 10(4), and 10(7) cells when administered by the intravenous, intraperitoneal, and subcutaneous routes, respectively. Virulence of the a...

متن کامل

Production of Pasteurella pestis toxin.

In the course of the development of a casein hydrolyzate mineral glucose medium for the growth of Pasteurella pestis, strain A1122, for the production of the envelope antigen (Englesberg and Levy, 1954), the potential use of this medium for plague toxin production became apparent. The development of a simplified method for the production of plague toxin made possible by this medium will be desc...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of bacteriology

دوره 96 6  شماره 

صفحات  -

تاریخ انتشار 1968